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fluorescent oligonucleotides - Oligonucleotides are polymer sequences of...

Thu, 16 Sep 2021 05:42:09 GMT

fluorescent oligonucleotides - Oligonucleotides are polymer sequences of nucleotides (RNA, DNA and their analogues). Health diagnosis and drug gene research were carried out by using primers and siRNA, oligonucleotides for basic molecular research, as well as microarray and fluorophore probes. Fluorophore is a very useful biomolecule sensitive marker, and fluorescent labeling is the most commonly used synthetic oligonucleotide marker so far. Many different fluorescent groups can be easily connected to oligonucleotides, which form the basis of the detection systems used in DNA sequencing, forensic medicine, and genetic analysis.

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enzyme labeling oligonucleotides - Enzyme-protein conjugates, or cross-linked...

Sat, 08 May 2021 03:19:51 GMT

enzyme labeling oligonucleotides - Enzyme-protein conjugates, or cross-linked enzymes, a highly sensitive and specific molecular probe is prepared by connecting it to an antibody or antigen protein molecule in the form of covalent bond and ionic bond. It is widely used in analytical research and technical determination in the fields of medical pathology, immunohistochemistry, molecular biology, biopharmaceutical and veterinary medicine. A typical example is the binding of "activated" horseradish peroxidase (HRP) to target antibodies, proteins in immunoassay. On the other hand, as a carrier-free immobilization technology, the cross-linking of enzyme molecules can expand the industrial enzyme pool by increasing the stability during use and storage. Coupling reactions with proteins or peptides are usually carried out by small molecular connectors (such as glutaraldehyde) or enzymes (such as transglutaminase), also known as cross-linking.

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enzyme labeling oligonucleotides - Enzyme-protein conjugates, or cross-linked...

Thu, 26 Nov 2020 13:15:38 GMT

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